1 Serum Factor Requirements for the Initiation of Cellular Proliferation. - Multiple Serum Components Required for Cellular Proliferation. - EOF and Somatomedin C Can Replace the GQ/GI Progession Activity of PPP. - EGF and Somatomedin C Reduce the Minimum Gi Transit Time. - Availability of Free Somatomedin C Controls Minimum Gi Transit Time. - Somatomedin C Regulates Progression of Late Gi Phase and Commitment to DNA Synthesis. - EGF Is Required during Traverse of Early Gi. - Summary and Conclusions. - References. - 2 Regulation of Fibroblast Growth by Multiple Growth Factors in Serum-Free Medium. - In Vitro Models of Growth RegulationWhy Do Cells Arrest Their Growth?. - Neoplastic Transformation and Relaxed Growth Regulation. - Replacing Serum with Defined Medium Components to Study Growth Regulation In Vitro. - Combinations of Growth Factors Are Required in Defined Media to Support Cell Proliferation. - Mechanism of Growth Factor ActionEvents Stimulated by Growth Factors Prior to DNA Synthesis. - Comparison of the Growth Factor Requirements of Normal and Transformed Cells Grown in Serum-Free Media. - Conclusion. - References. - 3 Growth and Differentiation of Preadipocyte Cell Lines in Serum-Free Medium. - Results and Discussion. - References. - 4 Growth and Differentiation of Human Myelomonocyte Leukemia Cell Lines in Serum-Free Medium. - Human Myelomonocytic Cell Lines. - Growth of HL-60 in Defined Medium. - Differentiation of HL-60 in Defined Medium. - Involvement of Intracellular Cyclic Adenosine 3?:5?-Monophosphate (cAMP) in Differentiation of HL-60. - Differentiation Effects of Agents That Increase Intracellular cAMP on HL-60 Primed with RA. - Growth and Differentiation of Other Myelomonocytic Cell Lines in Defined Medium. - References. - 5 In Vitro Immunization and Growth ofHybridomas in Serum-Free Medium. - In Vitro Immunization. - The Role of Serum. - Thiols. - Cellular Interactions and Soluble Factors. - Serum-Free Media. - Conclusion. - References. - 6 Kidney Cell Cultures in Hormonally Defined Serum-Free Medium. - Kidney Cell Culture in Serum-Supplemented Medium: MDCK and LLC-PKl as Model Systems. - Advantages of Kidney Cell Culture. - Limitations on Transport Studies with Kidney Cell Culture Imposed by Serum. - Limitations of Studies with Established Kidney Cell Lines: Importance of Primary Kidney Cell Culture. - Primary Kidney Culture and Hormonally Defined Medium. - Primary Culture of Specific Kidney Cell Types. - Use of Hormonally Defined Serum-Free Medium to Grow Specific Cell Types in the Kidney: The Concept of Selective Media. - Use of Primary Kidney Cell Culture to Study Medically Related Problems of the Kidney. - References. - 7 Rat Hepatocytes in Culture: A Model for Studies of Growth Control during Experimental Chemical Hepatocarcinogenesis. - Control of DNA Synthesis in Cultures of Adult Rat Hepatocytes. - Factors Required for the Induction of DNA Synthesis. - Platelet-Derived Growth Factor as an Inhibitor of Normal Hepatocyte DNA Synthesis. - Stimulatory Activity for Normal Hepatocyte DNA Synthesis in the Serum from Cancer Patients and Carcinogen-Fed Rats. - Altered Response of Hepatocytes from Carcinogen-Treated Rats to PDGF. - Discussion. - References. - 8 Intratesticular Regulation: Evidence for Autocrine and Paracrine Control of Testicular Function. - Testicular Cell Culture. - Autocrine Regulation of Testicular Cells. - Cell-Cell Interaction in the Testis. - Summary and Model of Intratesticular Regulation. - References. - 9 Attachment Factors in Cell Culture. - Collagen. - Fibronectin. - Laminin. - Chondronectin. - Serum-Spreading Factor. - Epibolin. - Fetuin. - Extracellular Matrix in Cell Culture. - Future Trends: Studies of Cell Shape and Cell-Substratum Interactions. - References. - 10 Cell Culture Studies Using Extracts of Extracellular Matrix to Study Growth and Differentiation in Mammalian Cells. - Background on Extracellular Matrix. - Cell Culture Methods Using Crude Extracts of Extracellular Matrix. - The Future. - References. Language: English